Predict metagenome function from 16S data
bio-microbiome-functional-predictionskillsetup L2★381
majiayu000/claude-skill-registry ↗What it does
Predict metagenomic function from 16S marker genes using PICRUSt2 pipeline
Best for
Metagenomics when shotgun sequencing is unavailable and KEGG/MetaCyc function prediction suffices.
Inputs
- · 16S ASV table (samples×taxa)
- · FASTA sequences
Outputs
- · KEGG orthologs (KO) abundance table
- · MetaCyc pathway predictions
- · NSTI quality scores
Requires
- · picrust2 cli
- · R phyloseq/ALDEx2
Preconditions
16S rRNA ASV calls; Ollama/picrust2 installed
Failure modes
High NSTI (>2) reduces prediction reliability; horizontal gene transfer not detected
Trust signals
- · NSTI quality-control thresholds documented
- · Pathway name mapping via KEGGREST