Design batch-aware experiments

Design balanced batch experiments

Biologists designing multi-batch sequencing to make batch effects correctable

• · Sample metadata (condition, sex, batch size)
• · Blocking and balance columns

• · Balanced batch assignment
• · SVA surrogate variables estimate

• · R (sva, designit)
• · limma

• · R environment set up
• · Sample metadata available

• · R not installed
• · Insufficient samples for balance
• · Confounded design detected

• · osat optimization ensures balance
• · Detects and estimates hidden batch effects