cyberneticlibrary

Find biological pathways in gene lists

pathway-enrichmentskillsetup L227,559
K-Dense-AI/scientific-agent-skills
What it does

Run ORA or GSEA to identify enriched biological pathways in gene lists

Best for

Interpreting DE genes, CRISPR hits, or cluster markers into biological pathways

Inputs
  • · gene list or ranked gene scores
  • · gene-set library (GO, KEGG, MSigDB)
Outputs
  • · enrichment table
  • · p-values, FDR, NES scores
  • · dotplot visualization
Requires
  • · gseapy
  • · g:Profiler
  • · Enrichr
  • · MSigDB
  • · KEGG
Preconditions

Gene symbols are valid; background universe chosen

Failure modes

Wrong method chosen (ORA vs GSEA); gene-ID mapping failed; multiple-testing correction ignored

Trust signals
  • · ORA/GSEA decision tree provided
  • · background universe guidance
  • · setup/install instructions precise