Aggregate histone mark maps across multiple studies
histone-aggregationskillsetup L3★35
ammawla/encode-toolkit ↗What it does
Aggregate histone ChIP-seq data across replicates and conditions
Best for
Creating robust consensus histone modification profiles by aggregating multiple ChIP-seq replicates
Inputs
- · peak files (narrowPeak/broadPeak)
- · bigwig signal files
Outputs
- · merged peak set
- · aggregated signal tracks
Requires
- · bedtools
- · deeptools
- · MACS2
Preconditions
Aligned BAM files or peak calls from MACS2
Failure modes
- · Replicate QC issues
- · Batch effects not removed
- · Signal normalization artifacts
Trust signals
- · Standard tools (bedtools, deeptools, MACS2)