Integrate GTEx tissue expression with regulatory data

Integrate GTEx tissue expression data with ENCODE regulatory elements

Validating that genes near ENCODE regulatory elements are actually expressed in the matching tissue

• · ENCODE enhancer peaks
• · gene ID (Ensembl)
• · tissue name

• · median TPM by tissue
• · transcript-level expression
• · eQTL associations

• · GTEx REST API (gtexportal.org/api/v2)

Ensembl gene IDs for matching (not HGNC symbols)

• · Gene not expressed in bulk GTEx
• · Enhancer-gene distance assumptions wrong
• · Cell-type specificity hidden by bulk data

• · GTEx v8 (54 tissues, 948 donors)
• · GTEx Consortium 2020 Nature publication
• · 54 tissue panel